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H.LouiseJ.SalterA.BeachS.et al (2023)

Indoxyl sulfate has been described to raise the cytosolic Ca 2+ concentration and to stimulate erythrocyte cell membrane scrambling, thus amplifying the PS exposure on the surface of RBCs [253]

Lysis was achieved through five to six iterative freeze-thaw cycles: frozen samples were thawed at 23 C in a thermomixer (Thermomixer Comfort, Eppendorf) until 60-80% liquefied, then held on ice until fully thawed, before being rapidly refrozen in liquid nitrogen

The therapeutic compounded form should be physician-directed and pharmacy-sourced, not bought from gray-market suppliers

Reconstitution errors

A causative role of oxidative stress in ALD is supported by several lines of evidence 27