8.3 Clinical decision matrix: stratification by metabolic phenotype and reproductive timeline 8.3.1 Dimensions Vertical Axis: High Risk: BMI 30 kg/m 2 comorbid with IR, MASLD, impaired glucose tolerance (IGT), or a significant family history of cardiovascular disease
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This is the same mechanism that makes all diets increasingly difficult over time, and it applies to tirzepatide-assisted weight loss as well
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Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere
