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The membranes were blocked for 60 min, followed by incubation with either mouse anti-TUBB monoclonal antibody (Sigma-Aldrich, RRID: AB_477577) or rabbit anti-HOXC8 monoclonal antibody (Abcam, Tokyo, Japan, RRID: AB_1925078) at a 1:1000 dilution

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Over decades, free radicals accumulate, damaging DNA, proteins, and cell membranes