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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

The two cysteine residues at positions 7 and 14 form an intramolecular disulfide bond that creates a small cyclic loop within the 16-residue chain

Jul 2019;36(7):1379-1385

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Furthermore, SAXS is currently efficiently combined with other techniques, such as NMR, to provide a more comprehensive structural picture of flexible systems, such as a tumor suppressor p53 (Wells et al., 2008)

Resources: Pickart, L., & Margolina, A