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coenzymes bind more loosely to the enzyme, and are thus modified during the enzymatic reaction, while prosthetic groups are more tightly bound to the enzyme and are not modified

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The lower FI observed at pH 5, 7, and 9 suggest a less compact protein structure, where aromatic and hydrophobic residues are far apart and engaged in more interactions with the hydrophilic environment (Du et al., 2022)

Murakami S, Kondo Y, Sakurai T, Kitajima H, Nagate T

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