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(2) Cross-nutrient common mechanistic gaps and future research directions Across all nutrient categories, three critical gaps in mechanistic understanding persist and must be addressed in future research: Quantification of microbiota-metabolite-depression thresholds: for example, the minimum amount of dietary fiber required to elevate fecal butyrate concentrations to levels that alleviate depressive symptoms remains unclear
Frozen hind paws were used for the estimation of lipid peroxides (malondialdehyde, MDA), nitric oxide (NO), reduced glutathione (GSH), TNF-alpha level and HO-1 messenger RNA (mRNA) expression
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Funding No specific source of funding is associated with this work
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