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Red cells have a much higher concentration of folate than the serum, so measuring folate on a haemolysed sample would tell you nothing

Maintenance doses are then tailored to keep those levels topped up, and their duration of effect is directly tied to the prescribed frequency (e.g., a shot every 12 weeks for many)

doi: 10.4084/MJHID.2020.043

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Freeze-thaw cycles degrade the peptide and should be avoided