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Attention to supporting factors often determines the difference between good and excellent results
However, significant challenges remain, including the synthesis of stable tripeptides with good tissue penetration, insufficient understanding of their mechanisms of action, the scarcity of studies in larger animal models and human trials, and the lack of direct head-to-head comparisons with larger peptides [75, 95]
Whole-cell biocatalyst The GshF-overexpressing cells were obtained by centrifugation of the induced culture at 12,000 rpm and 4 C for 5 min, followed by two washes with 50 mM phosphate buffer (pH 7.0)
This process can involve gut microbiota metabolites crossing the blood-brain barrier, modulating the microbiota-gut-brain axis, and regulating microglial activity and cytokine release
(Figure 15)
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