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Cells were incubated overnight at 4C, followed by three washes in ice cold 1 PBS to remove unbound radioligand

The change in maximum [Ca 2+ ]i upon the addition of each concentration of anamorelin was determined in quadruplicate (n = 4 wells)

Graphical Abstract Similar content being viewed by others Introduction Bariatric surgery is the most effective therapy for severe obesity

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Avgerinos et al

Latebreaking now at #ADA2019Oral semaglutide versus subcutaneous liraglutide and placebo in type 2 #diabetes (#PIONEER4): a randomised, double-blind, phase 3a trial hubs.ly/H0jdgvr0 Latebreaking now at #ADA2019Oral semaglutide versus subcutaneous liraglutide and placebo in type 2 #diabetes (#PIONEER4): a randomised, double-blind, phase 3a trial hubs.ly/H0jdgvr0