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The reconstituted solution must be refrigerated
Then pellets were resuspended in lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail (Thermo Scientific, 1861280), thoroughly vortexed for 1 min and incubated for 15 min on ice
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Diagnostic and Statistical Manual of Mental Disorders
Human clinical-trial data (Phase 1/2 studies in wound healing, cardiac injury, and dry eye) exist only for a different, full-length recombinant thymosin beta-4 product, not for the short synthetic fragment marketed as TB-500, so no completed published human trial has evaluated TB-500 specifically for muscle, tendon, or joint recovery
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