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Cell culture and imaging HeLa cells were cultured in minimum essential medium containing 10% fetal bovine serum and antibiotics (100 units/mL penicillin and 100 g/mL streptomycin) in a 5% CO 2 humidity incubator at 37C

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62 GI disorders are attributed to humoral imbalances, particularly involving Safra (linked to bile and liver function) and Balgham (associated with mucus and cold conditions)