Tomita and Castillo, 2012
These periods can last 12 hours up to several days - an extreme side of this diet
Briefly, for SNAP/FLAG-hGLP-1R gold labelling, INS-1 832/3 SNAP/FLAG-hGLP-1R cells grown on Thermanox coverslips (Agar Scientific), or mouse islets transduced with SNAP/FLAG-hGLP-1R adenoviruses, were labelled with 2 M cleavable SNAP-Surface biotin probe (kind gift of Dr Ivan Corra Jr, New England Biolabs) in full media, followed by incubation with 5 g/mL NaN 3 -free Alexa Fluor 488 Streptavidin, 5 or 10 nm colloidal gold conjugate (Thermo Fisher Scientific) to label SNAP/FLAG-hGLP-1R prior to stimulation with 100 nM exendin-4, fixation and processing for conventional TEM
RuY and RoY checked the content of the manuscript
Grondin R, Bdard PJ, Hadj Tahar A, Grgoire L, Mori A, Kase H (1999) Antiparkinsonian effect of a new selective adenosine A2A receptor antagonist in MPTP-treated monkeys
But it amazes me how little to nothing they know about it Ok lets you a bit educated so at least you dont look clueless when you talk about it