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doi: 10.2174/1570159x13666160502153022

To generate the triple AA mutant with all three serine doublets mutated to alanine, the S441/2A mutant was used as a template to introduce serial alanine modifications at positions 451/452 and 444/445 using mutagenesis primers specifically designed to preserve the previous serine to alanine mutations

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, 39- , -4 ( ), -1 in vitro [29]

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