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After incubation (10 min, 39C), the plates were centrifuged (200 x g, 5 min, 20C, Universal 320, Hettich, Tuttlingen, Germany) and the medium was replaced with pre-warmed XF Seahorse medium supplemented with glucose, sodium pyruvate and glutamine (all purchased from Agilent, Santa Clara, California, USA)

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Therefore, an aging-associated lipolysis decrease may be related to changes in ATGL levels, but this theory requires further animal investigations

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Arokiyaraj S, Balamurugan R, Augustian P