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PMID 35987379

There would rightly, be an almighty uproar if every anaemic patient were told that they needed exactly the same amount of iron only 4 times per year, and all diabetic patients were told they need the same dose of insulin quarterly

Thats why careful experiments spread doses across at least three orders of magnitude to find the sweet spot, which moves based on the copper-to-peptide ratio, the buffer used, and how much serum sits in the media

Quick Scientific Profile Importantly, the EC50 values came from cell-based transactivation assays

Rodrigues, M., Kosaric, N., Bonham, C

[18] GAB1 is a key coordinator of the cellular responses to MET and binds the MET intracellular region with high avidity, but low affinity