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This disbelief and misjudgment (40, 58) delays any possible early diagnosis for the patients and has also resulted in psychologisation of the disease (40, 53)

Following 24, 48, 72, or 96 h after seeding, the medium was supplemented with 10 L of CCK-8 reagent (Beyotime) and the cells were incubated at 37 C for 2 h