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Cells were dissociated with Accutase and resuspended in PBS with 3% BSA

In this context, glutaminase 1 (GLS1), expressed in the mitochondria of several malignant tumors (ovarian cancer, breast tumor cells, colorectal cancer), promotes glutamine degradation to support cellular proliferation [200,201,202]

doi: 10.1016/j.biopha.2020.110195 303 ZhangR.SunC.ChenX.HanY.ZangW.JiangC.et al

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