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After confirming that the concentration of NADPH in our lysates was within the linear detection range of the assay (Fig 3A), we treated LPS-stimulated (6 h) RAW264.7 Ms with 2-DG and diamide and quantified NADPH kinetics by obtaining lysates at 5-minute intervals for a total of 25 minutes (Fig 3B)

If Methylcobalamin is left out of the fridge after being mixed it will not damage the medication

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The aorta was fixed in 4% fixative for 3 h, followed by thorough removal of surrounding tissues

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Rev Bra Eng Agrc Ambient 21:156162