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The metabolites were annotated using the KEGG database and statistical analysis was performed using the statistical software R (R version R-3.4.3), Python (Python 2.7.6 version), and CentOS (CentOS release 6.6)

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Functional in vitro characterization demonstrated that BiSpekDAb bound IL-23 and TNF- with EC50 values of 11.39 2.7 nM and 12.72 8.25 nM, respectively

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Methanol molecules were only built at sites where a water molecule did not fully abolish the difference Fourier density upon subsequent refinement cycles and where the carbon atom of the methanol molecule would not exist within 3.3 of any other nonbonded atoms