The slides were mounted with Fluoprep (BioMerieux), and immunofluorescence was viewed and analyzed using an Imager Z1 fluorescence microscope equipped with a Digital Camera (Zeiss, Jena, Germany) connected to an AxioVs40 software (Zeiss), and with an LMS800 confocal microscope piloted by a ZEN 2.3 software (Zeiss)
Selenium status, for instance, differs dramatically by geography: populations in low-selenium regions may benefit from targeted intake, whereas those in replete environments risk toxicity with supplementation (123, 199)
doi: 10.1182/blood-2015-02-631044
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Aliquoting into sterile, low-bind tubes for storage and use
Int J Rad Oncol Biol Phys 120(2):e457