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This might possibly be due to the engineered control circuits responding to the target product intermediate, limiting any improvement in production beyond initial control levels

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Its more likely to occur if you have a history of pancreatitis or drink alcohol

Cells were incubated with an anti-calreticulin antibody (1:200, Abcam, ab2907) or anti-calnexin antibody (1:1000, Abcam, ab22595), diluted in blocking buffer for 1 h in order to counterstain the ER

Before each injection, hold the pen up to the light and examine the liquid through the viewing window