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Proteins were separated using SDS-PAGE, and transferred to polyvinylidene fluoride (PVDF) membranes (#1620177, Bio-Rad, USA)

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Therefore, even when the main effect of the use of Lt in wines from areas affected by global warming is on the control of pH and alcoholic degree, a positive modulation of aroma can also be obtained by the formation of positive floral and fruity esters or the release of bonded thiols and terpenes

If an ordinary balanced salt or buffered saline solution is used to dilute the OptiPrep, then these two densities are equivalent to approx 6.5% and 14% (w/v) iodixanol, i.e

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