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Also known as MT-1, Melanotan 1 is a synthetic peptide designed to stimulate melanin production in the skin, offering a potential tan without prolonged sun exposure

Mass Spectrometric Detection (MS/MS) Each fragment is analyzed to determine its mass and sequence, enabling precise structural characterization

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In brief, trypsinized cells were washed three times with PBS and lysed in lysis buffer (5 mM triethanolamine, 150 mM NaCl, 4% SDS, 2 units benzonase, 5 mM PMSF, pH 7.4) with protease inhibitor cocktail followed by adding EDTA to a final concentration of 5 mM