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Absorbance at 600 nm was measured using a BioTek Synergy Neo-2 plate reader at 37C with cycles of 7 min shaking and 3 min rest for 15 hours

In an invited commentary on this study, the authors discussed the mixed literature on the impact of GLP-1 receptor agonists and risks of developing or progressing AMD, and raised some concerns regarding this latest research

GHK-Cuis a smallpeptidebound to copper (Cu), renowned for its regenerative and anti-aging properties

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For cell stimulation, cells were pretreated with various concentrations of GHK-Cu of 1, 5, or 10 M for 18 hours, followed by stimulation with 100 ng/ml LPS for specific time periods