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Figure 6: Percentage of Nissl-positive neurons in each of the four different experimental groups included in the first part of the study

It occurs when the liver no longer processes bilirubin, leading to its accumulation in the bloodstream

Analytes (M-CuP or D-CuP) were diluted in PBST buffer (10 mM phosphate-buffered saline, 0.05% Tween-20, pH 7.4) and injected at increasing concentrations at a flow rate of 30 L/min, with 120 s association and 180 s dissociation phases

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Monitoring results, tweaking doses, and troubleshooting issues requires consistent attention over protocol duration