Epub 2010 Jun 5
A good example of this is given by flutamide, an antiandrogen used in the treatment of prostatic carcinoma, which undergoes substantial metabolism in vivo with hydrolysis of the amide bond liberating 3-trifluoromethyl-4-nitroaniline
After an additional twenty-four hours, cells were removed from the incubator, placed on ice, lysed (without a PBS wash) with 1 mL of ice cold 80% methanol/20% water solution containing 1.5 M metabolomics amino acid mix (Cambridge Isotope Laboratories, MSK-A2-1.2)
The original online version of this Article was revised: In the original version of this Article, Affiliation 1 was incorrectly given as Department of Internal Medicine, Hospital Universitari Germans Trias i Pujol, Universitat Autnoma de Barcelona, 08916 Badalona, Spain
Baldini G, Phelan KD
Reason: Stress increases free-radical activity, and poor sleep reduces the body's ability to repair cells and produce antioxidants