A shift toward green or brown indicates copper speciation change, likely reduction to copper(I) or formation of copper oxides
This isn't done just for show
Peptides are not intended for human or animal use of any kind
Add the clear sample containing target GST-tagged protein onto the equilibrated gravity column, keep the sample in column for at least 2 min, ensure that the sample fully binds with the resin, and collect the flowthrough with a flow-rate of 0.5 - 1 mL per minute, then add the flowthrough onto the column again, repeat the previous operation, this can increase the binding efficiency
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