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While some pens may appear to have leftover medicine, this small amount is either used for flow testing (Ozempic) or is a slight manufacturing overfillnot intended for use as an extra dose

However, according to the Ozempic SmPC, clinical studies have not demonstrated significant reductions in the efficacy of commonly tested medications

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

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