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Changes in gene expression were determined by normalizing mRNA levels to those of GAPDH as an internal control and fold change was calculated using the 2 CT method

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NAC activates cystine-glutamate antiport transporters present in astrocytes, leading to the release of glutamate that stimulates extra-synaptic metabotropic receptors (mGluRs), thereby reducing the synaptic release of the neurotransmitter (Baker et al., 2002