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The binding of HGF to c-met was detected with biotinylated anti-HGF polyclonal Ab (1:500 dilution in the 0.1% BSA-TTBS for 3 h at 25C), TACS streptavidin-HRP conjugate (1:1600 dilution for 20 min), and TMB substrate solution

Funding This work was funded by the Natural Science Foundation of Gansu (22JR11RA021), Gansu Province Health Industry Project (GSWSKY2022-11), Fund of the First Hospital of Lanzhou University (ldyyyn2023-14) and Student Innovation and Entrepreneurship Action Plan of Lanzhou University (20220060247)

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Acta 1830 , 49995005 (2013)