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Once reticulocytes enter the circulation, they lose their intracellular organelles (ribosomes, mitochondria, etc) within about 24 h, becoming young red blood cells and concomitantly losing their ability to synthesize protein

Fretz T, Tanawuttiwat T, Ilonze OJ, Das MK

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After reconstitution with bacteriostatic water, refrigerate and use within 30 to 45 days

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Additionally, ferroptosis-related genes were also differentially expressed, among which the up-regulation of GPx4 , FTH1 , and SLC3A2 might be attributed to the activation of the negative feedback regulation mechanism of cancer cells