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Western blot analysis Cell extracts were prepared with RIPA buffer containing proteases and phosphatase inhibitors and the concentration of cell lysates was determined using the Bradford protein assay reagent (Bio-Rad)

AsGGT2-Core-F (5-CTCCTCCACATTAATGGAAC-3) and AsGGT2-Core-R (5-AAGTGGTCCCAAACATTTGTC-3) for AsGGT2

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Meanwhile, changes in body weight at week 68 for obesity or overweight with comorbidity after a 20-week run-in resulted in greater proportions of patients treated with Wegovy achieving 5%, 10%, and 15% weight loss than those treated with placebo

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