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The exact mechanism of eNOS modification is unknown but in vivo experiments have shown that eNOS activation in aortas and iNOS transgenetic expression in mouse heart both result in NO-induced protein S-glutathionylation

For the preparation of mitochondrial subfractions, 1 mg of isolated mitochondria was suspended with 40 l T10E20 buffer (10 mM Tris-HCL, 1 mM EDTA, pH 7.6) and then incubated with digitonin (0.1 mg digitonin/mg of mitochondria protein) for 10 min on ice

[PubMed] [Google Scholar] 81.Gatti S, Faggioni R, Echtenacher B, Ghezzi P

PMID: 21209017

Journal of the International Society of Sports Nutrition, 12 (Suppl 1), P41

For people primarily concerned with gut integrity, intestinal inflammation, or ulcers, oral administration provides direct contact with the tissues that need support