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Change You May Notice B12 Injection Oral Supplement More energy About 1 to 2 days Several weeks Mental clarity About 3 to 5 days 4 to 6 weeks Mood support About 1 to 2 weeks 1 to 2 months What Affects How Quickly a B12 Shot Works for You If you are comparing notes with a friend and wondering how fast a B12 shot works for them versus you, a few things explain the difference: Your starting levels