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Although Sorafenib is a kinase inhibitor with multiple targets including RTKs and RAF, its treatment did not affect the total tyrosyl phosphorylation and ERK phosphorylation in TamR cells (Figure S5), indicating that inhibition of TamR ER+ breast cancer cell growth by Sorafenib under the concentration used is not through inhibition of RTKs and RAF

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Each freeze-thaw cycle on reconstituted IGF-1 LR3 reduces potency by 3050% due to ice crystal formation that mechanically denatures the protein structure