When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr
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It has been studied in laboratory research for its role in skin, tissue, and cellular support
Then, D-CuP loadedROS- responsive hydrogel was prepared and the preparation process was illustrated in Fig
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PDRN (Polydeoxyribonucleotide): the adenosine A2A receptor agonist PDRN is a mixture of short and long DNA polymers extracted, in commercial preparations, from the gametes of salmon trout