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DNA was immunoprecipitated from the sonicated cell lysates with the anti-Nrf2 antibody (Cell Signaling Technology, 12721) and quantified using qRT-PCR

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Primer-dependent and de novo (unprimed) initiation of RNA synthesis have been demonstrated for this protein

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Contraindications : Avoid use in research models with known hypersensitivity to peptides or in studies lacking proper ethical oversight