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Tesaglitazar was covalently attached to the side chain amine of the C-terminal lysine 40 residue via a gamma glutamic acid spacer (Extended Data Fig

Cells were harvested by centrifugation, frozen, and lysed with BugBuster (Merck KGaA), supplemented with 2 mg/mL lysozyme (Roche)

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However, natural production often declines due to age, stress, and environmental factors

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