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For experiments involving oxygen control, purified CD8 + T cells were immediately acclimatized to the required oxygen tension (21%, 5% or 1% O 2 ) for 2 h in complete RPMI 1640 supplemented with 10% FBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, before activation with anti-CD3/CD28 Dynabeads and 30 U ml 1 IL-2

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Glutathione and Cysteine: Glutathione is a low-molecular-weight thiol-tripeptide, primal to the maintenance of intracellular redox balance: it is an antioxidant present in almost every cell in the body, playing a role in the detoxification of xenobiotics, drugs and pollutants

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M., Rucker, R