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3 College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, Jeddah, 22384, Saudi Arabia

However, despite this limitation, it is possible to successfully demonstrate oxidative modifications of these proteins by immunoprecipitation with a specific antibody against each target protein and then immunoblot analysis with a specific antibody against S-NO-Cys or glutathione (for detecting mixed disulfides)

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At first, you may need injections every other day for a couple of weeks or until symptoms start improving