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The protein pellet was analyzed using Pierce BCA Protein Assay kit (Thermofisher) to determine protein concentrations, and the supernatant was transferred into a new tube for the identification of metabolite levels by mass spectrometry analysis at the Metabolomics Expertise Center (MEC - KU Leuven/VIB)

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The role of sulfur-containing amino acids in superoxide production and modification of low density lipoprotein by arterial smooth muscle cells

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The product itself exceeded expectations