A80-229AP, Bethyl Laboratories, Inc.), diluted in PBS/T/M (pH 5.5 or pH 7.4), was added for 1 h at room temperature, followed by a washing step and detection with 1 mg/mL (100 L/well) of p -Nitrophenyl Phosphate Substrate (Sigma-Aldrich) dissolved in diethanolamine buffer (pH 9.8) (Sigma-Aldrich)
It takes 30 seconds total and costs nothing
J., Thorburn, A., Ogru, E., Gianello, R., Jiang, W
Gain: 160, Manual
Understanding endometriosis and why current treatments fall short Endometriosis is a chronic inflammatory condition where tissue similar to the uterine lining grows outside the uterus
in contrast, subclinical deficiency is common and may present with hypophosphatemia, hypocalcemia, secondary hyperparathyroidism, bone demineralization, and an increased risk of fractures