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However, under reducing conditions, the cobalt center is reduced to Co(II) or even Co(I), which are usually denoted as B 12r and B 12s , for reduced and super reduced respectively

So there could be germs on top of the bottle that we dont want to get in the vial because what is inside the vial is considered sterile

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Step 6: Gently Introducing the Water to the Peptide This is the most delicate part of the entire process

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