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The plasmid DNA was linearised with the restriction enzyme Spe1 (New England BioLabs, Inc) and transcribed into mRNA by T7 RNA polymerase with the AMBION mMESSAGE mMACHINE TM T7 transcription kit (Invitrogen TM , Thermo Fisher Scientific)

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(1) K Raun, B S Hansen, N L Johansen, et al (1998) Ipamorelin, the first selective growth hormone secretagogue European Journal of Endocrinology, 1998 Nov, Volume 139 (Issue 5), Pages 552-61