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Briefly, cells labeled with alkynyl probes were washed with ice-cold D-PBS [2.67 mM KCl, 138 mM NaCl, 1.47 mM KH 2 PO 4 , 8.1 mM Na 2 HPO 4 , pH 7.4] and lysed in lysis buffer [50 mM Tris-HCl [pH 7.4], 150 mM NaCl, 0.1% Triton X-100, 0.1% SDS] supplemented with protease inhibitor cocktail, 1 M palmostatin B and 50 mM NEM

Jing Xue, Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, China Updates Copyright 2022 Zhao, Hu, Xiao, Zhang, Zhao and Wang

Oregon State University, Micronutrient Information Center