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J Diabetes Metab Disord 25 , 44 (2026)

Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min

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Alternatively, as Lp glu is an intermediate formed by the OADHc that converts 2-oxoadipate to glutaryl-CoA, persistent glutarylation of the OGDHc-E2 would prevent the formation of glutaryl-CoA by the OADHc, and also render the OGDHc inactive