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Modified one-step real-time PCR Reactions were performed in a final volume of 50 l using the Superscript III Platinum one-step quantitative RT-PCR system (Invitrogen)

37 , D159D162 (2009)

The duration of treatment depends on the patient's reaction and medical criteria

As hypothesized, we found the level of total GSH, free GSH, and GSSG to be significantly reduced in the EDTA-treated plasma stored at 4 o C compared to the non-deproteinized EDTA-treated plasma stored at 80 o C (Preparation Condition 3)

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Ferroptosis-inducing factors can directly or indirectly affect glutathione peroxidase through different pathways, resulting in a decrease in antioxidant capacity and accumulation of lipid reactive oxygen species (ROS) in cells, ultimately leading to oxidative cell death