Front Endocrinol (Lausanne) 12:1
ML226 reduced oxidative phosphorylation, as expected, but prolonged inhibition of ABHD11 was required to induce this reduction, as short-term treatment of CD8 + T cells (24 h) did not alter the oxygen consumption rate (OCR
Following incubation, caspase 3 activity was measured using the fluorogenic substrate AC-DEVD-AMC as described before ( 2 , 1.2 mM EGTA, 1.5% Nonidet P40, 0.3% CHAPS, 30% sucrose, 150 M caspase 3 substrate DEVD-AMC, 30 mM DTT, and 3.0 mM PMSF was directly added to HepG2 cells
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The cycle threshold (CT) values of gene expression were normalized to the housekeeping gene -actin, and the expression ratios were calculated using the CT method