Here we determined the rate constants for the individual redox reactions between the two redox-sensitive cysteinyl residues of roGFP2 and the active-site cysteinyl residues of the model class I glutaredoxin PfGrx from the malaria parasite Plasmodium falciparum using stopped-flow kinetic measurements in combination with redox mobility shift assays
doi: 10.1089/ars.2012.4581 PubMed Abstract | CrossRef Full Text | Google Scholar D'Incalci, M., Steward, W
One microgram of RNA was reverse-transcribed to single stranded cDNA using the RevertAid H minus First Strand cDNA Synthesis Kit (Thermo Fisher, USA)
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