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The protein was eluted with buffer containing 20 mM HEPES, pH 7.5, 500 mM NaCl, 0.1% (w/v) DDM, 0.02% (w/v) CHS, 400 mM imidazole and 1 M PF-05190457 and loaded onto an anti-Flag M1 antibody resin after adding 2 mM CaCl 2

On Day 3, the speed of rotarod was gradually escalated from 4 rpm to an astonishing 40 rpm within a mere span of 5 min and each mouse would test for three times

BouillonRMarcocciCCarmelietGBikleDWhiteJHDawson-HughesBet al

Telomere length in granulosa cells and leukocytes: a potential marker of female fertility

Hetzer SM, Guilhaume-Correa F, Day D, Bedolla A, Evanson NK (2021) Traumatic optic neuropathy is associated with visual impairment, neurodegeneration, and endoplasmic reticulum stress in adolescent mice

"Anti-obesity drug discovery: advances and challenges"